Respiratory syncytial virus leads to 33.1 infections and 60,000 in-hospital deaths each year in children under 5 years. The most at-risk demographic to severe RSV disease are infants born preterm, being more likely to require hospitalisation, admission to the intensive care unit and ventilatory assistance. The reason for their increased susceptibility is multi-factorial, however, a major contributor is thought to be an immature immune system. In this study, we have collected cord blood from 25 preterm infants and 25 term infants and stimulated the cord blood mononuclear cells with RSVA or RSVB for 24 and 96h to observe innate and adaptive immune responses. Multiplex assays and flow cytometry were performed at both timepoints whilst RNA-sequencing was only performed at 24h. We found a consistently elevated inflammatory response in term infants following RSVA stimulation through cytokine secretion (IL-1β, IL-6 and IL-17A) chemokine secretion (IL-8 and eotaxin) and gene expression (XCL1, CXCL1, CXCL2, GZMB, NLRP6, IL-1β and IL1R1). Flow cytometry revealed a higher proportion of transitional B-cells, Th2 and Treg populations in preterm infants suggesting promotion of anti-inflammatory immune responses. Overall, preterm infants exhibit a diminished inflammatory response that may contribute to their increased susceptibility to severe RSV disease. These data may aid the development of therapeutics or vaccines to reduce the burden of disease in this highly vulnerable group.